STUDY ON THE EFFECT OF TEMPERATURE ON THE CALCINATION TREATMENT TECHNIQUE FOR OBTAINING SILICOPHYTOLITH
DOI:
https://doi.org/10.5710/PEAPA.01.08.2023.470Keywords:
Phytoliths, Morphometry, Arundo donax L, Bilobate, CalcinationAbstract
This paper presents the analysis of the variation in size and shape of BILOBATE silicophytolith in leaves of Arundo donax L. processed by calcination at different temperature ranges (specifically at 500 ºC, 650 ºC, 800 ºC, and 950 ºC). The morphometric variables used were size and shape (e.g., length, width, perimeter, area, convex perimeter, convex area, shape factor, roundness, convexity, solidity, and aspect ratio). The phytolith association for this species was characterized by the presence of ELONGATE morphotypes, BULLIFORM FLABELLATE, BILOBATE, POLYLOBATE, CROSS, and stomatal structures, both unicellular and multicellular. The phytolith variability of leaves processed at 950 ºC showed a lower number of CROSS and BILOBATE and a greater abundance of ELONGATE and BULLIFORM FLABELLATE silicophytoliths. Meanwhile, the number of articulated BILOBATE phytoliths decreased and the articulated BULLIFORM FLABELLATE phytoliths increased. The BILOBATE morphotypes were selected for morphometric analysis because of their high frequency. When processed at 950 ºC, they show color and shape variations, indicated by high values of convexity, solidity, shape factor and roundness, and size (represented by perimeter reduction). These results would indicate a trend in the change in size, expressed as a reduction in the perimeter, and a variation in shape, related to greater sphericity and convexity of the morphotypes. Complementary observations were made by SEM and SEM-EDS analysis, showing silica loss in silicophytoliths processed at 950 ºC. The present work contributes to the methodological knowledge of processing plant material for silicophytolith extraction.
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